Blutwerte · Marker

Isoprostane

8-Isoprostane · F2-Isoprostanes · Lipid Peroxidation Marker

Longevity & Biohacking

Einheit:: ng/mL
Abbreviation: 8-iso-PGF2a

Isoprostanes measure the level of lipid peroxidation, indicating oxidative stress in the body.

Reference Ranges

Reference

0.1–0.5ng/mL

0.1

0.5

LowNormalHigh

Reference

Unit · ng/mL

Reference ranges may vary based on laboratory methods and individual factors.

Overview

Übersicht

Isoprostanes, specifically 8-iso-PGF2a, are prostaglandin-like compounds formed through the non-enzymatic peroxidation of arachidonic acid, a polyunsaturated fatty acid. These molecules are generated independently of the cyclooxygenase pathway and serve as reliable biomarkers of oxidative stress, reflecting lipid peroxidation in the body. Researchers have identified various isoprostane species, including those derived from other polyunsaturated fatty acids, which have expanded the understanding of oxidative processes in human health. Clinically, elevated levels of isoprostanes have been associated with numerous conditions characterized by oxidative stress, such as obesity, ischemia-reperfusion injury, central nervous system disorders, cancer, and genetic diseases. Their presence in biological fluids like urine and plasma makes them accessible for non-invasive measurement, providing insights into the oxidative status of individuals. For athletes and biohackers, monitoring isoprostane levels can be crucial in assessing the balance between oxidative stress and antioxidant defenses, which is vital for optimizing performance and longevity. However, researchers note that various factors, including diet, lifestyle, and individual metabolic differences, can influence isoprostane levels. Additionally, the timing of sample collection and potential confounding factors like recent physical activity or dietary intake should be considered when interpreting results.

Klinische Bedeutung
Elevated isoprostane levels indicate increased oxidative stress, which is associated with various chronic diseases and conditions. Reduced levels may suggest lower oxidative stress or effective antioxidant defenses.

Dynamics

Trend Interpretation

Rising Values

Progressively rising values suggest increasing oxidative stress, warranting lifestyle and dietary interventions. Retest in 4-6 weeks if elevated.

Falling Values

Progressively falling values indicate reduced oxidative stress, potentially due to effective interventions.

Re-test Interval

4 weeks if outside optimal range

Etiology

Causes — High & Low

Cause

Elevated Levels

Oxidative stress
Chronic inflammation
Poor diet
Environmental toxins
Smoking
Excessive alcohol consumption

Cause

Low Levels

Effective antioxidant defenses
Healthy diet
Regular physical activity
Low oxidative stress

Protocol

How to Optimize

Lever

Lifestyle

Regular exercise
Stress management
Adequate sleep

Lever

Nutrition

Increased intake of fruits and vegetables
Omega-3 fatty acids
Whole grains

Lever

Supplementation

Vitamin E
Vitamin C
Coenzyme Q10

Note:

Consult a healthcare provider before starting any supplementation, especially if on medication.

Testing Guidelines

✓Fasting Not Required

✓Not Time-Sensitive

Collect samples at the same time of day to ensure consistency.

Testing Frequency

Annually for general health monitoring, more frequently if managing oxidative stress-related conditions.

Interfering Factors

Recent intense exercise
Dietary intake
Smoking

Related Peptides & Hormones

Interleukin 6

Correlated with

hormone or peptide

Open Research Questions

Current research suggests that the biological activity of isoprostanes and their specific reference ranges in various populations remain understudied, particularly in veterinary medicine. Researchers have not yet established optimal targets for isoprostane levels in different disease contexts or addressed potential confounders affecting their measurement. Additionally, clinical questions regarding the therapeutic implications of modulating isoprostane levels in chronic diseases and the role of diet in influencing these biomarkers remain unanswered.

47 Research Publications

2,467

Total Citations

Human/RCT

4.6

Avg. Influence

2021

Latest

Sort

Filter

01Top

#01

Quantification of isoprostanes as indices of oxidant stress and the risk of atherosclerosis in humans.

ReviewInfluence17.0

458

View on PubMed

02Top

#02

The isoprostanes--25 years later.

Milne Ginger L, et al. · Biochimica et biophysica acta · 2015

ReviewInfluence7.0

326

This review examined the role of isoprostanes (IsoPs) as biomarkers of oxidative stress in various diseases, including obesity and cancer. Researchers found that IsoPs are generated from lipid peroxidation and can be influenced by diet and lifestyle factors. The study highlights the significance of IsoPs in clinical research over the past 25 years.

Key findings

01IsoPs are produced from the oxidation of fatty acids and are important markers of oxidative stress.
02They have been linked to various health conditions, including obesity and cancer.
03Diet and lifestyle choices can affect the natural levels of IsoPs in the body.

View on PubMed

03Top

#03

Clinical perspective on oxidative stress in sporadic amyotrophic lateral sclerosis.

ReviewInfluence12.0

295

This review explored the role of oxidative stress in sporadic amyotrophic lateral sclerosis (ALS). Researchers observed that oxidative stress biomarkers are elevated in ALS patients, indicating a significant contribution to motor neuron degeneration. The study suggests that multiple environmental factors may exacerbate oxidative stress and ALS progression.

View on PubMed

#04

Urinary biomarkers of oxidative status.

ReviewInfluence15.0

248

This review focused on urinary biomarkers of oxidative status, emphasizing their potential in studying chronic diseases. Researchers found that F₂-isoprostanes are recommended for monitoring oxidative stress over time, providing a non-invasive method to assess redox balance in populations.

View on PubMed

#05

Classifying oxidative stress by F2-isoprostane levels across human diseases: A meta-analysis.

Meta-AnalysisInfluence5.0

159

This meta-analysis classified oxidative damage levels using F2-isoprostanes across various human diseases. Researchers found that elevated levels of 8-iso-PGF2α were associated with conditions like chronic renal insufficiency and cystic fibrosis, indicating significant oxidative stress. The study provides a quantitative framework for assessing oxidative damage in health and disease.

View on PubMed

#06

The therapeutic potential of phytoestrogens.

ReviewInfluence1.0

115

Researchers investigated the effects of phytoestrogens on oxidative damage, noting their potential to decrease plasma F₂-isoprostane concentrations. The study suggests that phytoestrogens may contribute to antioxidant activity, which could be beneficial in reducing the risk of chronic diseases.

View on PubMed

#07

F2-isoprostanes in Alzheimer and other neurodegenerative diseases.

ReviewInfluence2.0

109

This review focused on the role of F₂-isoprostanes in Alzheimer's disease and other neurodegenerative conditions. Researchers found that increased levels of F₂-isoprostanes in the brain correlate with oxidative stress and may aid in diagnosing Alzheimer's disease.

View on PubMed

#08

Recent advances in the biochemistry and clinical relevance of the isoprostane pathway.

Review

107

View on PubMed

#09

Urinary 8-isoprostane as a biomarker for oxidative stress. A systematic review and meta-analysis.

Graille M, et al. · Toxicology letters · 2020

Meta-AnalysisInfluence2.0

This systematic review and meta-analysis assessed urinary 8-isoprostane as a biomarker for oxidative stress. Researchers found that 8-isoprostane levels vary significantly among individuals and are influenced by factors like body mass index. The study recommended standardized methods for measuring this biomarker to improve comparability across studies.

Key findings

01Researchers observed that the average urinary 8-isoprostane concentration varies based on BMI, with higher levels found in individuals with a higher BMI.
02The study included 63 research articles, providing a comprehensive analysis of 8-isoprostane levels in healthy adults.
03Researchers recommend using specific chemical methods for measuring 8-isoprostane and adjusting results for creatinine to allow for better comparison across studies.

View on PubMed

#10

Systematic review on the association between F2-isoprostanes and cardiovascular disease.

Meta-AnalysisInfluence5.0

This systematic review assessed the association between F2-isoprostanes and cardiovascular disease (CVD). Researchers found that high levels of F2-isoprostanes were significantly associated with CVD in most studies reviewed. However, the review highlights the need for more population-based studies to clarify their role as biomarkers for CVD.

122

Started

2010

Primary outcome

a change in plasma F-2-isoprostane concentration

Chronic Kidney Disease

Study of Docosahexanoic Acid in Patients With Cystic Fibrosis (CF)

NCT02690857Phase 2COMPLETED

Sponsor

Hospices Civils de Lyon

Enrollment

Started

2010

Primary outcome

Change in 8-isoprostane level in urine.

Cystic Fibrosis

Association Between Increased Oxidative Stress, Anti-Inflammatory Fatty Acid Formation, and Airway Infection in People With Asthma and Chronic Obstructive Pulmonary Disease

NCT00595114COMPLETED

Sponsor

Brigham and Women's Hospital

Enrollment

Started

2007

Primary outcome

8-isoprostane Levels as Biochemical Markers for Nonenzymatic Oxidative Stress in Asthma

AsthmaPulmonary Disease, Chronic Obstructive

Non-Interventional F-Two Isoprostane Trial (NIFTI)

NCT00505908COMPLETED

Sponsor

University of Texas Southwestern Medical Center

Enrollment

Started

2007

Primary outcome

Levels of oxidant injury in cerebrospinal fluid following severe traumatic brain injury

Traumatic Brain Injury

The Comparison of Oxidative Stress Between Inhalation Anesthetics

NCT00965445COMPLETED

Sponsor

Chang Gung Memorial Hospital

Enrollment

Started

2007

Primary outcome

isoprostanes isomer as marker of oxidative stress

Oxidative StressCardiac Surgery

Propofol Cardioprotection for Type II Diabetics

NCT00734383Phase 2/3COMPLETED

Sponsor

University of British Columbia

Enrollment

137

Started

2005

Primary outcome

Perioperative Plasma 15 f2t isoprostane, a biologically active marker of oxidative stress

Myocardial Injury

Publication Trend

Research publications about Isoprostane over time

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