Nerve Growth Factor (NGF), also known as Beta-NGF, is an endogenous protein primarily produced in the nervous system and reproductive tracts of various mammals. It belongs to the neurotrophin family, which plays a crucial role in the development and maintenance of the nervous system. NGF is synthesized by both neuronal and non-neuronal tissues, including mesenchymal stem/stromal cells (MSCs) and reproductive organs. Researchers have extensively studied its role in neural development and its presence in seminal plasma. NGF's primary physiological roles include promoting neuronal survival, differentiation, and sensory innervation. It is also involved in immunoregulation and tissue repair, particularly in the context of inflammation and hyperalgesia. In reproductive biology, NGF has been identified as an ovulation-inducing factor in camelids and is present in the seminal plasma of bulls and stallions, where it may influence reproductive processes. The mechanism of action of NGF involves binding to its high-affinity receptor TrkA and the low-affinity receptor p75NTR. This interaction triggers a cascade of intracellular signaling pathways that regulate cell survival, differentiation, and immune responses. In MSCs, NGF binding to TrkA is crucial for their survival and angiogenic functions. Pharmacokinetic properties of NGF, such as its half-life and metabolism, are not well-documented in the literature, and its bioavailability by various routes remains largely unknown. Clinically, NGF is not widely used as a therapeutic agent, and its regulatory status varies by region. Researchers continue to explore its potential applications in regenerative medicine and reproductive health, but it is not yet approved for specific clinical uses.